Prognostic impact of mRNA levels of osteopontin splice variants in soft tissue sarcoma patients.

ABSTRACT:

Background

It is well known that osteopontin (OPN) plays an important role in tumor progression and that a high OPN expression level in several tumor entities correlates with poor prognosis in cancer patients. However, little is known about the prognostic relevance of the OPN mRNA splice variants.

Methods

We analyzed the mRNA expression levels of different OPN splice variants in tumor tissue of 124 soft tissue sarcoma (STS) patients. Quantitative real-time PCR (qRT-PCR) was used to analyze the mRNA expression level of three OPN splice variants (OPN-a, -b and -c).

Results

The multivariate Cox's proportional hazard regression model revealed that high mRNA expression levels of OPN splice variants are significantly associated with poor prognosis in STS patients (n = 124).

SUBMITTER: Hahnel A 

PROVIDER: S-EPMC3364873 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature