Real-time determination of intracellular oxygen in bacteria using a genetically encoded FRET-based biosensor.

ABSTRACT:

Background

Molecular oxygen (O2) is one of the key metabolites of all obligate and facultative aerobic pro- and eukaryotes. It plays a fundamental role in energy homeostasis whereas oxygen deprivation, in turn, broadly affects various physiological and pathophysiological processes. Therefore, real-time monitoring of cellular oxygen levels is basically a prerequisite for the analysis of hypoxia-induced processes in living cells and tissues.

Results

We developed a genetically encoded Förster resonance energy transfer (FRET)-based biosensor allowing the observation of changing molecular oxygen concentrations inside living cells. This biosensor named FluBO (fluorescent protein-based biosensor for oxygen) consists of the yellow fluorescent protein (YFP) that is sensitive towards

SUBMITTER: Potzkei J 

PROVIDER: S-EPMC3364895 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature