Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.

ABSTRACT: We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak interactions. Conversion of ligands into SNAP-tag substrates thus provides access to a powerful toolbox for the analysis of their interactions with proteins.

SUBMITTER: Haruki H 

PROVIDER: S-EPMC3365113 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature