Robust quantification of polymerase chain reactions using global fitting.

ABSTRACT:

Background

Quantitative polymerase chain reactions (qPCR) are used to monitor relative changes in very small amounts of DNA. One drawback to qPCR is reproducibility: measuring the same sample multiple times can yield data that is so noisy that important differences can be dismissed. Numerous analytical methods have been employed that can extract the relative template abundance between samples. However, each method is sensitive to baseline assignment and to the unique shape profiles of individual reactions, which gives rise to increased variance stemming from the analytical procedure itself.

Principal findings

We developed a simple mathematical model that accurately describes the entire PCR reaction profile using only two reaction variables that depict the maximum capacity of

SUBMITTER: Carr AC 

PROVIDER: S-EPMC3365123 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature