Superpenetration optical microscopy by iterative multiphoton adaptive compensation technique.

ABSTRACT: Biological tissues are rarely transparent, presenting major challenges for deep tissue optical microscopy. The achievable imaging depth is fundamentally limited by wavefront distortions caused by aberration and random scattering. Here, we report an iterative wavefront compensation technique that takes advantage of the nonlinearity of multiphoton signals to determine and compensate for these distortions and to focus light inside deep tissues. Different from conventional adaptive optics methods, this technique can rapidly measure highly complicated wavefront distortions encountered in deep tissue imaging and provide compensations for not only aberration but random scattering. The technique is tested with a variety of highly heterogeneous biological samples including mouse brain tissue, skull

SUBMITTER: Tang J 

PROVIDER: S-EPMC3365222 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature