Peptidomics approach to elucidate the proteolytic regulation of bioactive peptides.

ABSTRACT: Peptide hormones and neuropeptides have important roles in physiology and therefore the regulation of these bioactive peptides is of great interest. In some cases proteolysis controls the concentrations and signaling of bioactive peptides, and the peptidases that mediate this biochemistry have proven to be extremely successful drug targets. Due to the lack of any general method to identify these peptidases, however, the role of proteolysis in the regulation of most neuropeptides and peptide hormones is unknown. This limitation prompted us to develop an advanced peptidomics-based strategy to identify the peptidases responsible for the proteolysis of significant bioactive peptides. The application of this approach to calcitonin gene-related peptide (CGRP), a neuropeptide associated with bloo

SUBMITTER: Kim YG 

PROVIDER: S-EPMC3365226 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature