Role of conserved glycine in zinc-dependent medium chain dehydrogenase/reductase superfamily.

ABSTRACT: The medium-chain dehydrogenase/reductase (MDR) superfamily consists of a large group of enzymes with a broad range of activities. Members of this superfamily are currently the subject of intensive investigation, but many aspects, including the zinc dependence of MDR superfamily proteins, have not yet have been adequately investigated. Using a density functional theory-based screening strategy, we have identified a strictly conserved glycine residue (Gly) in the zinc-dependent MDR superfamily. To elucidate the role of this conserved Gly in MDR, we carried out a comprehensive structural, functional, and computational analysis of four MDR enzymes through a series of studies including site-directed mutagenesis, isothermal titration calorimetry, electron paramagnetic resonance (EPR), quantum me

SUBMITTER: Tiwari MK 

PROVIDER: S-EPMC3365981 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature