The GPCR-associated sorting protein 1 regulates ligand-induced down-regulation of GPR55.

ABSTRACT:

Background and purpose

Many GPCRs, including the CB(1) cannabinoid receptor, are down-regulated following prolonged agonist exposure by interacting with the GPCR-associated sorting protein-1 (GASP-1). The CB(1) receptor antagonist rimonabant has also recently been described to be an agonist at GPR55, a cannabinoid-related receptor. Here we investigated the post-endocytic properties of GPR55 after agonist exposure and tested whether GASP-1 is involved in this process.

Experimental approach

We evaluated the direct protein-protein interaction of GPR55 with GASP-1 using (i) GST-binding assays and (ii) co-immunoprecipitation assays in GPR55-HEK293 cells with endogenous GASP-1 expression. We further tested the internalization, recycling and degradation of GPR55 using confocal fluo

SUBMITTER: Kargl J 

PROVIDER: S-EPMC3366063 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature