West Nile virus: characterization and diagnostic applications of monoclonal antibodies.

ABSTRACT:

Background

Diagnosis of West Nile virus (WNV) infections is often difficult due to the extensive antigenic cross-reactivity among flaviviruses, especially in geographic regions where two or more of these viruses are present causing sequential infections. The purpose of this study was to characterize a panel of monoclonal antibodies (MAbs) produced against WNV to verify their applicability in WNV diagnosis and in mapping epitope targets of neutralizing MAbs.

Methods

Six MAbs were produced and characterized by isotyping, virus-neutralization, western blotting and MAb-epitope competition. The MAb reactivity against various WNVs belonging to lineage 1 and 2 and other related flaviviruses was also evaluated. The molecular basis of epitopes recognized by neutralizing MAbs was defi

SUBMITTER: Lelli D 

PROVIDER: S-EPMC3366889 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature