A novel strategy for selection and validation of reference genes in dynamic multidimensional experimental design in yeast.

ABSTRACT:

Background

Understanding the dynamic mechanism behind the transcriptional organization of genes in response to varying environmental conditions requires time-dependent data. The dynamic transcriptional response obtained by real-time RT-qPCR experiments could only be correctly interpreted if suitable reference genes are used in the analysis. The lack of available studies on the identification of candidate reference genes in dynamic gene expression studies necessitates the identification and the verification of a suitable gene set for the analysis of transient gene expression response.

Principal findings

In this study, a candidate reference gene set for RT-qPCR analysis of dynamic transcriptional changes in Saccharomyces cerevisiae was determined using 31 different publicly av

SUBMITTER: Cankorur-Cetinkaya A 

PROVIDER: S-EPMC3366934 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature