High resolution clustering of Salmonella enterica serovar Montevideo strains using a next-generation sequencing approach.

ABSTRACT:

Background

Next-Generation Sequencing (NGS) is increasingly being used as a molecular epidemiologic tool for discerning ancestry and traceback of the most complicated, difficult to resolve bacterial pathogens. Making a linkage between possible food sources and clinical isolates requires distinguishing the suspected pathogen from an environmental background and placing the variation observed into the wider context of variation occurring within a serovar and among other closely related foodborne pathogens. Equally important is the need to validate these high resolution molecular tools for use in molecular epidemiologic traceback. Such efforts include the examination of strain cluster stability as well as the cumulative genetic effects of sub-culturing on these clusters. Numerous isol

SUBMITTER: Allard MW 

PROVIDER: S-EPMC3368722 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature