Complex nested promoters control tissue-specific expression of acetyl-CoA carboxylase genes in wheat.

ABSTRACT: Cis-acting regulatory elements of the wheat acetyl-CoA carboxylase (ACC) gene family were identified by comparing the promoter activity of 5' end gene fragments fused to a reporter gene in two transient expression systems: wheat protoplasts and epidermal cells of mature embryos. Expression of the plastid and the cytosolic ACC genes is each driven by two nested promoters responsible for the synthesis of two transcript types. The internal promoter is located in an intron removed from transcripts originating at the first promoter. These complex promoters, which are different for the cytosolic and plastid ACC genes, control tissue-specific expression of the enzymatic activity supplying cytosolic, plastid, and mitochondrial pools of malonyl-CoA. The activity of one such complex promoter, drivin

SUBMITTER: Zuther E 

PROVIDER: S-EPMC337065 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature