Detection of drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of iran.

ABSTRACT:

Background

Cutaneous leishmaniasis is still a health problem in many rural and urban regions of Iran and drug resistance has emerged as a major impediment in the treatment of leishmaniasis. This study aims to determine the drug resistance gene in cutaneous leishmaniasis by PCR in some endemic areas of Iran.

Methods

Ninety seven samples were collected from ulcers of leishmaniasis patients from some endemic areas of Iran. The Giemsa stained samples were examined microscopically and cultured in NNN and RPMI 1640 mediums for parasite detection. After DNA extraction, PCR was done by a pair of specific primers. For detection of mutation in DNA, first PCR products were electrophoresed on CSGE gel. The suspected samples were compared by sequencing and RFLP results were demonstrated.

SUBMITTER: Alizadeh R 

PROVIDER: S-EPMC3371903 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature