Genetic interleukin-10 deficiency causes vascular remodeling via the upregulation of Nox1.

ABSTRACT:

Background and hypothesis

Interleukin (IL)-10 is an anti-inflammatory cytokine. Nox1 is a mitogenic oxidase (p65-mox). The objective of this study was to test a hypothesis that IL-10 deficiency would cause vascular remodeling via the upregulation of Nox1.

Methods and results

Recombinant adeno-associated virus (AAV) carrying short hairpin small interference RNA for Nox1 (AAV.Nox1shRNA) was constructed for in-vivo-specific inhibition of Nox1. Three groups of IL-10 gene knockout (IL-10KO) mice and three groups of wild-type mice were used. Three groups of each strain received intravenous delivery of AAV.Nox1shRNA, AAV with scrambled shRNA, and PBS, respectively. Animals were euthanized at 3 weeks after gene delivery. IL-10KO increased Nox1 protein expression, NADPH oxidase activ

SUBMITTER: Dammanahalli JK 

PROVIDER: S-EPMC3373265 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature