Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography.

ABSTRACT: Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of atleast 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins’ functions were obtained from their interactions with each of a panel of ligands.

SUBMITTER: Kim H 

PROVIDER: S-EPMC3375012 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature