The development of three long universal nuclear protein-coding locus markers and their application to osteichthyan phylogenetics with nested PCR.

ABSTRACT:

Background

Universal nuclear protein-coding locus (NPCL) markers that are applicable across diverse taxa and show good phylogenetic discrimination have broad applications in molecular phylogenetic studies. For example, RAG1, a representative NPCL marker, has been successfully used to make phylogenetic inferences within all major osteichthyan groups. However, such markers with broad working range and high phylogenetic performance are still scarce. It is necessary to develop more universal NPCL markers comparable to RAG1 for osteichthyan phylogenetics.

Methodology/principal findings

We developed three long universal NPCL markers (>1.6 kb each) based on single-copy nuclear genes (KIAA1239, SACS and TTN) that possess large exons and exhibit the appropriate evolutionary rates. We

SUBMITTER: Shen XX 

PROVIDER: S-EPMC3375249 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature