Structural modeling of TAL effector-DNA interactions.

ABSTRACT: TAL (transcriptional activator-like) effectors are DNA-binding repeat proteins recently shown to recognize their target sites by an unprecedented, 1:1 mapping between repeat residues and DNA bases. The structural basis for this recognition is not known; in particular, it is not clear whether such 1:1 recognition can be accommodated by standard major-groove readout of B-form DNA. Here we describe a structure prediction protocol tailored to the TAL-DNA system, and report simulation results that shed light on observed repeat-base associations and overall TAL structure. Our models demonstrate that TAL-DNA interactions can be explained by a model in which the TAL repeat domain forms a superhelical repeat structure that wraps around undistorted B-form DNA, paralleling the geometry of the major g

SUBMITTER: Bradley P 

PROVIDER: S-EPMC3375747 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature