Selective fluorescence detection of cysteine and N-terminal cysteine peptide residues.

ABSTRACT: A new fluorogenic fluorescein derivative containing an alpha,beta-unsaturated aldehyde moiety produces a selective fluorescent signal enhancement in the presence of cysteine or peptides containing N-terminal cysteine residues. The mechanism is based on synergistic covalent and supramolecular interactions.

SUBMITTER: Lim S 

PROVIDER: S-EPMC3376164 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature