PER1 modulates SGLT1 transcription in vitro independent of E-box status.

ABSTRACT:

Background and aims

The intestine demonstrates profound circadian rhythmicity in glucose absorption in rodents, mediated entirely by rhythmicity in the transcription, translation, and function of the sodium glucose co-transporter SGLT1 (Slc5a1). Clock genes are rhythmic in the intestine and have been implicated in the regulation of rhythmicity of other intestinal genes; however, their role in the regulation of SGLT1 is unknown. We investigated the effects of one clock gene, PER1, on SGLT1 transcription in vitro.

Methods

Caco-2 cells were stably transfected with knockdown vectors for PER1 and mRNA expression of clock genes and SGLT1 determined using quantitative polymerase chain reaction (qPCR). Chinese hamster ovary (CHO) cells were transiently cotransfected with combination

SUBMITTER: Balakrishnan A 

PROVIDER: S-EPMC3376756 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature