Quantification of retrograde axonal transport in the rat optic nerve by fluorogold spectrometry.

ABSTRACT:

Purpose

Disturbed axonal transport is an important pathogenic factor in many neurodegenerative diseases, such as glaucoma, an eye disease characterised by progressive atrophy of the optic nerve. Quantification of retrograde axonal transport in the optic nerve usually requires labour intensive histochemical techniques or expensive equipment for in vivo imaging. Here, we report on a robust alternative method using Fluorogold (FG) as tracer, which is spectrometrically quantified in retinal tissue lysate.

Methods

To determine parameters reflecting the relative FG content of a sample FG was dissolved in retinal lysates at different concentrations and spectra were obtained. For validation in vivo FG was injected uni- or bilaterally into the superior colliculus (SC) of Sprague Dawl

SUBMITTER: van Oterendorp C 

PROVIDER: S-EPMC3377715 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature