High-throughput mutation profiling identifies frequent somatic mutations in advanced gastric adenocarcinoma.

ABSTRACT:

Background

Gastric cancer is one of the leading cancer types in incidence and mortality, especially in Asia. In order to improve survival, identification of a catalogue of molecular alterations underlying gastric cancer is a critical step for developing and designing genome-directed therapies.

Methodology/principal findings

The Center for Cancer Genome Discovery (CCGD) at the Dana-Farber Cancer Institute (DFCI) has adapted a high-throughput genotyping platform to determine the mutation status of a large panel of known cancer genes. The mutation detection platform, termed OncoMap v4, interrogates 474 "hotspot" mutations in 41 genes that are relevant for cancer. We performed OncoMap v4 in formalin-fixed paraffin-embedded (FFPE) tissue specimens from 237 gastric adenocarcinomas

SUBMITTER: Lee J 

PROVIDER: S-EPMC3377730 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature