Proteomic profiling of the effect of metabolic acidosis on the apical membrane of the proximal convoluted tubule.

ABSTRACT: The physiological response to the onset of metabolic acidosis requires pronounced changes in renal gene expression. Adaptations within the proximal convoluted tubule support the increased extraction of plasma glutamine and the increased synthesis and transport of glucose and of NH(4)(+) and HCO(3)(-) ions. Many of these adaptations involve proteins associated with the apical membrane. To quantify the temporal changes in these proteins, proteomic profiling was performed using brush-border membrane vesicles isolated from proximal convoluted tubules (BBMV(PCT)) that were purified from normal and acidotic rats. This preparation is essentially free of contaminating apical membranes from other renal cortical cells. The analysis identified 298 proteins, 26% of which contained one or more transmem

SUBMITTER: Walmsley SJ 

PROVIDER: S-EPMC3378174 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature