Upregulation of miR-196b confers a poor prognosis in glioblastoma patients via inducing a proliferative phenotype.

ABSTRACT:

Purpose

To explore the expression pattern, prognostic value and functional role of miR-196b in glioblastoma (GBM) patients using large cohorts.

Experimental design

MiR-196b expression was measured using the Human v2.0 miRNA Expression BeadChip (Illumina) in 198 frozen glioma tissues. The expression levels of miR-196b were also validated in an independent cohort containing 128 formalin-fixed paraffin-embedded (FFPE) glioma samples using qRT-PCR. The presence of other molecular prognostic indicators was assessed centrally in the glioma samples. Whole genome gene profiling was performed to investigate the underlying biological behavior. MiR-196b functional analyses were performed in U87 and U251 cell lines.

Results

The expression levels of miR-196b were inversely correla

SUBMITTER: Ma R 

PROVIDER: S-EPMC3378534 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature