A pilot study of rapid benchtop sequencing of Staphylococcus aureus and Clostridium difficile for outbreak detection and surveillance.

ABSTRACT:

Objectives

To investigate the prospects of newly available benchtop sequencers to provide rapid whole-genome data in routine clinical practice. Next-generation sequencing has the potential to resolve uncertainties surrounding the route and timing of person-to-person transmission of healthcare-associated infection, which has been a major impediment to optimal management.

Design

The authors used Illumina MiSeq benchtop sequencing to undertake case studies investigating potential outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile.

Setting

Isolates were obtained from potential outbreaks associated with three UK hospitals.

Participants

Isolates were sequenced from a cluster of eight MRSA carriers and an associated bacteraemia c

SUBMITTER: Eyre DW 

PROVIDER: S-EPMC3378946 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature