Alpha synuclein gene expression profile in the retina of vertebrates.

ABSTRACT:

Purpose

Alpha-synuclein is a Parkinson's disease-linked protein of ubiquitous expression in the central nervous system. It has a proposed role in the modulation of neurotransmission and synaptic function. This study was aimed at analyzing expression of the alpha-synuclein gene in the normal retina, and characterizing its pattern of distribution in the different retinal cell types and layers in a variety of vertebrates, ranging from fish to humans.

Methods

Reverse transcriptase-polymerase chain reaction and immunoblotting were used to assess alpha-synuclein expression at both mRNA and protein levels. Its retinal distribution profile was characterized by immunohistochemical methods. With this purpose, retinal sections were analyzed under fluorescent confocal microscopy using s

SUBMITTER: Martinez-Navarrete GC 

PROVIDER: S-EPMC3380420 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature