An efficient method for transient gene expression in monocots applied to modify the Brachypodium distachyon cell wall.

ABSTRACT:

Background

Agrobacterium-mediated transformation is widely used to produce insertions into plant genomes. There are a number of well-developed Agrobacterium-mediated transformation methods for dicotyledonous plants, but there are few for monocotyledonous plants.

Methods

Three hydrolase genes were transiently expressed in Brachypodium distachyon plants using specially designed vectors that express the gene product of interest and target it to the plant cell wall. Expression of functional hydrolases in genotyped plants was confirmed using western blotting, activity assays, cell wall compositional analysis and digestibility tests.

Key results

An efficient, new, Agrobacterium-mediated approach was developed for transient gene expression in the grass B. distachyon, using c

SUBMITTER: Fursova O 

PROVIDER: S-EPMC3380599 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature