In vivo effects of histone H3 depletion on nucleosome occupancy and position in Saccharomyces cerevisiae.

ABSTRACT: Previous studies in Saccharomyces cerevisiae established that depletion of histone H4 results in the genome-wide transcriptional de-repression of hundreds of genes. To probe the mechanism of this transcriptional de-repression, we depleted nucleosomes in vivo by conditional repression of histone H3 transcription. We then measured the resulting changes in transcription by RNA-seq and in chromatin organization by MNase-seq. This experiment also bears on the degree to which trans-acting factors and DNA-encoded elements affect nucleosome position and occupancy in vivo. We identified ∼60,000 nucleosomes genome wide, and we classified ∼2,000 as having preferentially reduced occupancy following H3 depletion and ∼350 as being preferentially retained. We found that the in vivo influence of DNA seque

SUBMITTER: Gossett AJ 

PROVIDER: S-EPMC3380831 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature