Intravital microscopy to study myocardial engraftment.

ABSTRACT: Tissue engineering and stem cell transplantation are promising novel therapies for myocardial repair. A major barrier to cell survival after transplantation involves inadequate vascularization. Continuous observation of cardiac tissue engraftment and angiogenesis could help understand these processes and allow for identification of the optimal conditions for these therapeutic interventions. We investigated the ability of a skin-fold chamber model to allow for engraftment of differentiated myocardial tissue in mice. Neonatal atrial and ventricular tissues were implanted in the in vivo chambers. All myocardial implants had a high rate of engraftment (86-95%). Tissue engraftment was preceded by a 'bleeding phase' in both the atrial and ventricular implants. This occurred earlier in ventricula

SUBMITTER: Lushaj EB 

PROVIDER: S-EPMC3380977 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature