Chimeric glutathione S-transferases containing inserts of kininogen peptides: potential novel protein therapeutics.

ABSTRACT: The study of synthetic peptides corresponding to discrete regions of proteins has facilitated the understanding of protein structure-activity relationships. Short peptides can also be used as powerful therapeutic agents. However, in many instances, small peptides are prone to rapid degradation or aggregation and may lack the conformation required to mimic the functional motifs of the protein. For peptides to function as pharmacologically active agents, efficient production or expression, high solubility, and retention of biological activity through purification and storage steps are required. We report here the design, expression, and functional analysis of eight engineered GST proteins (denoted GSHKTs) in which peptides ranging in size from 8 to 16 amino acids and derived from human high

SUBMITTER: Bentley AA 

PROVIDER: S-EPMC3381172 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature