Mapping the Deltex-binding surface on the notch ankyrin domain using analytical ultracentrifugation.

ABSTRACT: The Notch signal transduction pathway controls cell fate determination during metazoan development. The Notch gene encodes a transmembrane receptor that is cleaved upon activation, liberating the Notch intracellular domain, which enters the nucleus and assembles transcriptional activation complexes that drive expression of Notch-responsive genes. The most conserved region of the Notch intracellular domain is an ankyrin domain (Nank), which binds directly to the cytosolic effector protein Deltex (Dx), controlling intracellular Notch activity. However, the structural and energetic basis for this interaction remains unknown. Here, we analyze the thermodynamics and hydrodynamics of the Nank:Dx heteroassociation, as well as a weaker Nank self-association, using sedimentation velocity analytical

SUBMITTER: Allgood AG 

PROVIDER: S-EPMC3381501 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature