Optimized in vivo transfer of small interfering RNA targeting dermal tissue using in vivo surface electroporation.

ABSTRACT: Electroporation (EP) of mammalian tissue is a technique that has been used successfully in the clinic for the delivery of genetic-based vaccines in the form of DNA plasmids. There is great interest in platforms which efficiently deliver RNA molecules such as messenger RNA and small interfering RNA (siRNA) to mammalian tissue. However, the in vivo delivery of RNA enhanced by EP has not been extensively characterized. This paper details the optimization of electrical parameters for a novel low-voltage EP method to deliver oligonucleotides (both DNA and RNA) to dermal tissue in vivo. Initially, the electrical parameters were optimized for dermal delivery of plasmid DNA encoding green fluorescent protein (GFP) using this novel surface dermal EP device. While all investigated parameters resulte

SUBMITTER: Broderick KE 

PROVIDER: S-EPMC3381603 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature