Production of isotopically labeled standards from a uniformly labeled precursor for quantitative volatile metabolomic studies.

ABSTRACT: Optimal accuracy and precision in small-molecule profiling by mass spectrometry generally requires isotopically labeled standards chemically representative of all compounds of interest. However, preparation of mixed standards from commercially available pure compounds is often prohibitively expensive and time-consuming, and many labeled compounds are not available in pure form. We used a single-prototype uniformly labeled [U-(13)C]compound to generate [U-(13)C]-labeled volatile standards for use in subsequent experimental profiling studies. [U-(13)C]-α-Linolenic acid (18:3n-3, ALA) was thermally oxidized to produce labeled lipid degradation volatiles which were subsequently characterized qualitatively and quantitatively. Twenty-five [U-(13)C]-labeled volatiles were identified by headspace

SUBMITTER: Gomez-Cortes P 

PROVIDER: S-EPMC3381853 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature