Bifidobacteria isolated from infants and cultured on human milk oligosaccharides affect intestinal epithelial function.

ABSTRACT:

Objectives

Human milk oligosaccharides (HMOs) are the third most abundant component of breast milk. Our laboratory has previously revealed gene clusters specifically linked to HMO metabolism in selected bifidobacteria isolated from fecal samples of infants. Our objective was to test the hypothesis that growth of selected bifidobacteria on HMO stimulates the intestinal epithelium.

Methods

Caco-2 and HT-29 cells were incubated with lactose (LAC)- or HMO-grown Bifidobacterium longum subsp infantis (B infantis) or B bifidum. Bacterial adhesion and translocation were measured by real-time quantitative polymerase chain reaction. Expression of pro- and anti-inflammatory cytokines and tight junction proteins was analyzed by real-time reverse transcriptase. Distribution of tight junc

SUBMITTER: Chichlowski M 

PROVIDER: S-EPMC3381975 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature