Lipidomic analysis of extracellular vesicles from the pathogenic phase of Paracoccidioides brasiliensis.

ABSTRACT:

Background

Fungal extracellular vesicles are able to cross the cell wall and transport molecules that help in nutrient acquisition, cell defense, and modulation of the host defense machinery.

Methodology/principal findings

Here we present a detailed lipidomic analysis of extracellular vesicles released by Paracoccidioides brasiliensis at the yeast pathogenic phase. We compared data of two representative isolates, Pb3 and Pb18, which have distinct virulence profiles and phylogenetic background. Vesicle lipids were fractionated into different classes and analyzed by either electrospray ionization- or gas chromatography-mass spectrometry. We found two species of monohexosylceramide and 33 phospholipid species, including phosphatidylcholine, phosphatidylethanolamine, phosphatidi

SUBMITTER: Vallejo MC 

PROVIDER: S-EPMC3382159 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature