Fluorescence-based phenotypic selection allows forward genetic screens in haploid human cells.

ABSTRACT: The isolation of haploid cell lines has recently allowed the power of forward genetic screens to be applied to mammalian cells. The interest in applying this powerful genetic approach to a mammalian system is only tempered by the limited utility of these screens, if confined to lethal phenotypes. Here we expand the scope of these approaches beyond live/dead screens and show that selection for a cell surface phenotype via fluorescence-activated cell sorting can identify the key molecules in an intracellular pathway, in this case MHC class I antigen presentation. Non-lethal haploid genetic screens are widely applicable to identify genes involved in essentially any cellular pathway.

SUBMITTER: Duncan LM 

PROVIDER: S-EPMC3382162 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature