Structure and regulon of Campylobacter jejuni ferric uptake regulator Fur define apo-Fur regulation.

ABSTRACT: The full regulatory potential of the ferric uptake regulator (Fur) family of proteins remains undefined despite over 20 years of study. We report herein an integrated approach that combines both genome-wide technologies and structural studies to define the role of Fur in Campylobacter jejuni (Cj). CjFur ChIP-chip assays identified 95 genomic loci bound by CjFur associated with functions as diverse as iron acquisition, flagellar biogenesis, and non-iron ion transport. Comparative analysis with transcriptomic data revealed that CjFur regulation extends beyond solely repression and also includes both gene activation and iron-independent regulation. Computational analysis revealed the presence of an elongated holo-Fur repression motif along with a divergent holo-Fur activation motif. This dive

SUBMITTER: Butcher J 

PROVIDER: S-EPMC3382491 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature