Antibody phage display libraries: contributions to oncology.

ABSTRACT: Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different purposes, including the recognition of tumors. Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any target on the surface or inside cancer cells, or even soluble markers found in patient serum. Many phage display derived binders targeting important tumor markers have been identified. Selection directed to tumoral cells' surfaces lead to the identification of unknown tumoral markers. Also the improvement of methods that require smaller amounts of cells has opened the possibility to use this approach on patient samples. Robust techniq

SUBMITTER: Dantas-Barbosa C 

PROVIDER: S-EPMC3382779 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature