PDX1 regulation of FABP1 and novel target genes in human intestinal epithelial Caco-2 cells.

ABSTRACT: The transcription factor pancreatic and duodenal homeobox 1 (PDX1) plays an essential role in pancreatic development and in maintaining proper islet function via target gene regulation. Few intestinal PDX1 targets, however, have been described. We sought to define novel PDX1-regulated intestinal genes. Caco-2 human intestinal epithelial cells were engineered to overexpress PDX1 and gene expression profiles relative to control cells were assessed. Expression of 80 genes significantly increased while that of 49 genes significantly decreased more than 4-fold following PDX1 overexpression in differentiated Caco-2 cells. Analysis of the differentially regulated genes with known functional annotations revealed genes encoding transcription factors, growth factors, kinases, digestive glycosidases,

SUBMITTER: Chen C 

PROVIDER: S-EPMC3383377 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature