Line-scanning particle image velocimetry: an optical approach for quantifying a wide range of blood flow speeds in live animals.

ABSTRACT:

Background

The ability to measure blood velocities is critical for studying vascular development, physiology, and pathology. A key challenge is to quantify a wide range of blood velocities in vessels deep within living specimens with concurrent diffraction-limited resolution imaging of vascular cells. Two-photon laser scanning microscopy (TPLSM) has shown tremendous promise in analyzing blood velocities hundreds of micrometers deep in animals with cellular resolution. However, current analysis of TPLSM-based data is limited to the lower range of blood velocities and is not adequate to study faster velocities in many normal or disease conditions.

Methodology/principal findings

We developed line-scanning particle image velocimetry (LS-PIV), which used TPLSM data to quantify pe

SUBMITTER: Kim TN 

PROVIDER: S-EPMC3383695 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature