Epigenetic regulation of the calcitonin gene-related peptide gene in trigeminal glia.

ABSTRACT:

Background

The neuropeptide calcitonin gene-related peptide (CGRP) plays a key role in migraine. CGRP gene expression involves an enhancer that is active in neurons, yet inactive in glia. In this report, we analyze epigenetic modifications that allow enhancer activation in glia.

Methods

DNA methylation and histone acetylation states were measured in rat and human- model cell lines and primary cultures of rat trigeminal ganglia glia. The functional consequence of altering the chromatin state was determined by quantitative measurements of both calcitonin (CT) and CGRP mRNAs.

Results

A hypermethylated CpG island flanking the enhancer was identified in glia and non-expressing cell lines. In addition, the chromatin was hypoacetylated. Treatment with the DNA methylation inh

SUBMITTER: Park KY 

PROVIDER: S-EPMC3383834 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature