Hybrids of a genetically engineered antibody and a carbon nanotube transistor for detection of prostate cancer biomarkers.

ABSTRACT: We developed a novel detection method for osteopontin (OPN), a new biomarker for prostate cancer, by attaching a genetically engineered single-chain variable fragment (scFv) protein with high binding affinity for OPN to a carbon nanotube field-effect transistor (NT-FET). Chemical functionalization using diazonium salts is used to covalently attach scFv to NT-FETs, as confirmed by atomic force microscopy, while preserving the activity of the biological binding site for OPN. Electron transport measurements indicate that functionalized NT-FET may be used to detect the binding of OPN to the complementary scFv protein. A concentration-dependent increase in the source-drain current is observed in the regime of clinical significance, with a detection limit of approximately 30 fM. The scFv-NT hybr

SUBMITTER: Lerner MB 

PROVIDER: S-EPMC3383883 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature