Low-frequency nevirapine resistance at multiple sites may predict treatment failure in infants on nevirapine-based treatment.

ABSTRACT:

Background

Resistance commonly arises in infants exposed to single-dose nevirapine (sdNVP) for prevention of mother to child transmission. Although K103N and Y181C are common following sdNVP, multiple other mutations also confer NVP resistance. It remains unclear whether specific NVP resistance mutations or combinations of mutations predict virologic failure in infants when present at low frequencies before NVP-based treatment.

Methods

Twenty sdNVP-exposed infants who were subsequently treated with NVP-based highly active antiretroviral therapy were examined. Pretreatment plasma samples were tested for the presence of NVP resistance mutations by allele-specific polymerase chain reaction for K103N and Y181C and ultradeep pyrosequencing (UDPS) for all primary NVP mutations. Vi

SUBMITTER: Lehman DA 

PROVIDER: S-EPMC3383885 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature