Efficient genetic modification and germ-line transmission of primordial germ cells using piggyBac and Tol2 transposons.

ABSTRACT: The derivation of germ-line competent avian primordial germ cells establishes a cell-based model system for the investigation of germ cell differentiation and the production of genetically modified animals. Current methods to modify primordial germ cells using DNA or retroviral vectors are inefficient and prone to epigenetic silencing. Here, we validate the use of transposable elements for the genetic manipulation of primordial germ cells. We demonstrate that chicken primordial germ cells can be modified in vitro using transposable elements. Both piggyBac and Tol2 transposons efficiently transpose primordial germ cells. Tol2 transposon integration sites were spread throughout both the macro- and microchromosomes of the chicken genome and were more prevalent in gene transcriptional units an

SUBMITTER: Macdonald J 

PROVIDER: S-EPMC3384192 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature