High-throughput molecular analysis from leftover of fine needle aspiration cytology of mammographically detected breast cancer.

ABSTRACT: We investigated whether residual material from diagnostic smears of fine needle aspirations (FNAs) of mammographically detected breast lesions can be successfully used to extract RNA for reliable gene expression analysis. Twenty-eight patients underwent FNA of breast lesions under ultrasonographic guidance. After smearing slides for cytology, residual cells were rinsed with TRIzol to recover RNA. RNA yield ranged from 0.78 to 88.40 µg per sample. FNA leftovers from 23 nonpalpable breast cancers were selected for gene expression profiling using oligonucleotide microarrays. Clusters generated by global expression profiles partitioned samples in well-distinguished subgroups that overlapped with clusters obtained using "biologic scores" (cytohistologic variables) and differed from clusters bas

SUBMITTER: Annaratone L 

PROVIDER: S-EPMC3384272 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature