A Toll-like receptor 3 agonist (polyI:C) elicits innate host responses in the spleen and lungs of chickens.

ABSTRACT: Double-stranded (ds) RNA interacts with host Toll-like receptor (TLR-3), leading to the induction of anti-viral host responses. The present study was designed to compare different routes of administration of a synthetic dsRNA (polyI:C) for induction of innate responses in chicken spleen and lungs. Chickens were treated with polyI:C via the aerosol, intra-air sac (i.a.s.), and intramuscular (IM) routes. Spleen and lungs were collected at 0, 2, 6, 12, and 24 h post-treatment and the expression of innate defence genes was quantified by real-time reverse transcriptase polymerase chain reaction (RT-PCR). There was an up-regulation of interferon (IFN)-β, TLR-3, and Toll/interleukin 1 receptor domain-containing adaptor protein inducing IFN-β (TRIF) at 6 h post-treatment in the spleen via IM admin

SUBMITTER: Parvizi P 

PROVIDER: S-EPMC3384288 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature