Transcriptional provirus silencing as a crosstalk of de novo DNA methylation and epigenomic features at the integration site.

ABSTRACT: The autonomous transcription of integrated retroviruses strongly depends on genetic and epigenetic effects of the chromatin at the site of integration. These effects are mostly suppressive and proviral activity can be finally silenced by mechanisms, such as DNA methylation and histone modifications. To address the role of the integration site at the whole-genome-scale, we performed clonal analysis of provirus silencing with an avian leucosis/sarcoma virus-based reporter vector and correlated the transcriptional silencing with the epigenomic landscape of respective integrations. We demonstrate efficient provirus silencing in human HCT116 cell line, which is strongly but not absolutely dependent on the de novo DNA methyltransferase activity, particularly of Dnmt3b. Proviruses integrated clos

SUBMITTER: Senigl F 

PROVIDER: S-EPMC3384319 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature