High-throughput single-molecule analysis of DNA-protein interactions by tethered particle motion.

ABSTRACT: Tethered particle motion (TPM) monitors the variations in the effective length of a single DNA molecule by tracking the Brownian motion of a bead tethered to a support by the DNA molecule. Providing information about DNA conformations in real time, this technique enables a refined characterization of DNA-protein interactions. To increase the output of this powerful but time-consuming single-molecule assay, we have developed a biochip for the simultaneous acquisition of data from more than 500 single DNA molecules. The controlled positioning of individual DNA molecules is achieved by self-assembly on nanoscale arrays fabricated through a standard microcontact printing method. We demonstrate the capacity of our biochip to study biological processes by applying our method to explore the enzym

SUBMITTER: Plenat T 

PROVIDER: S-EPMC3384352 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature