Intergenic Polycomb target sites are dynamically marked by non-coding transcription during lineage commitment.

ABSTRACT: Non-coding (nc) RNAs are involved both in recruitment of vertebrate Polycomb (PcG) proteins to chromatin, and in activation of PcG target genes. Here we investigate dynamic changes in the relationship between ncRNA transcription and recruitment of PcG proteins to chromatin during differentiation. Profiling of purified cell populations from different stages of a defined murine in vitro neural differentiation system shows that over 50% of regulated intergenic non-coding transcripts precisely correspond to PcG target sites. We designate these PcG recruiting elements as Transcribed Intergenic Polycomb (TIP) sites. The relationship between TIP transcription and PcG recruitment switches dynamically during differentiation between different states, in which transcription and PcG recruitment exclud

SUBMITTER: Hekimoglu-Balkan B 

PROVIDER: S-EPMC3384584 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature