Identification of kinases regulating prostate cancer cell growth using an RNAi phenotypic screen.

ABSTRACT: As prostate cancer progresses to castration-resistant disease, there is an increase in signal transduction activity. Most castration-resistant prostate tumors continue to express the androgen receptor (AR) as well as androgen-responsive genes, despite the near absence of circulating androgen in these patients. The AR is regulated not only by its cognate steroid hormone, but also by interactions with a constellation of co-regulatory and signaling molecules. Thus, the elevated signaling activity that occurs during progression to castration resistance can affect prostate cancer cell growth either through the AR or independent of the AR. In order to identify signaling pathways that regulate prostate cancer cell growth, we screened a panel of shRNAs targeting 673 human kinases against LNCaP pro

SUBMITTER: Whitworth H 

PROVIDER: S-EPMC3384611 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature