PiggyBac transposition into primordial germ cells is an efficient tool for transgenesis in chickens.

ABSTRACT: Transgenic birds embody one of the most potent and exciting research tools in biotechnology for agriculture, medicine, and model animals. To date, retrovirus- or lentivirus-mediated transgenesis has been established in chickens and quail. However, despite having a valid technique for viral transduction to achieve transgenic birds, many obstacles exist for practical applications because of relatively low and variable rates of germ-line transmission and transgenic offspring showing transgene silencing, as well as safety issues related to viral vector use. Thus, the generation of transgenic poultry by nonviral integration is a prerequisite for the introduction of biotechnology to practical applications. Herein, we show that a germ-line-competent chicken primordial germ-cell (PGC) line was est

SUBMITTER: Park TS 

PROVIDER: S-EPMC3386051 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature